Better than HBOT? Our review of $40 peptide spray: TB 500

What is TB 500?

To answer, we first need to mention its parent: Thymosin Beta 4

Thymosin Beta 4 (TB4) is a large, naturally occurring master protein made of 43 amino acids that signals our cells to repair tissue and heal wounds.

TB 500 is small fragment of TB4 containing just 7 amino acids (the 17th through 23rd links in the chain). When isolated, it was given the name TB-500.

Another fragment we use and have discussed in other posts is TB4 Frag 1–4.

It’s an even smaller, 4-amino-acid portion from the very beginning of the TB4 chain. It’s available as capsules but TB 500 is not.

Some labs use TB 500 synonymously with the full TB4 (that is a misnomer) so for clarity, this post will mention the fragment numbers specifically.

Full length TB4 directs broad biological tasks and is unstable, prone to breaking down even if injected.

TB-500 (17–23) fragment isolates a portion involved in signalling cells to heal tissues, via cell migration and the growth of new blood vessels.

It signals cells to build new capillary networks, bringing the oxygen and nutrients needed to repair damaged tissue.

Fragment 1-4 is also valuable to signal cells to decrease inflammation, lay repair framework and prevent tissue scarring or fibrosis.

If you’ve been following our journey, you know we regularly use the peptide capsules TB4 Frag (1-4).

More recently, we added Fragment 17-23 (TB 500) as a sublingual spray.

Using them together provides synergistic benefits:

• Fragment 1-4 optimizes the underlying healing environment

• Fragment 17-23 improves structural healing & blood delivery throughout the body and brain

Our observations

I began using Fragment 17-23 for tendon repair and unbelievably it healed my tennis elbow in 24 hours!

Even more notably though, my brain felt extremely calm the entire first day.

Ever since I began using it I’ve felt the same, very zen, zero worries!

I’m usually pretty calm, but this is an ongoing unshakable calm that I can’t attribute to anything else.

My ADHD has pleasantly decreased to a point I almost feel like a typical person!

I’m also enjoying having none of the body wide aches / pains I might normally feel, excellent GI health and no brain fog.

•••

For my daughter who has ASD and PANS:

– From the first day, she was undeniably calmer ~ Things that would normally set off some nervous dysregulation didn’t ruffle her feathers

– Within a few days she began pronouncing words more clearly and using more sophisticated language & interested in socializing

– Her focus on cognitive tasks (word games, puzzles, reading) has been surprisingly deeper with less distractibility and for 10x longer amounts of time!

– Without my suggestion, she’s coming up with many ways to entertain herself that do not involve screens (a truly remarkable change) ☺️

Why is it helping so much?

My daughter has done many hours of HBOT treatments in the past so I can confidently say:

This peptide is responsible for improvements that feel similar to – but at a greater magnitude than – what we saw with HBOT

For comparison

How HBOT works: By flooding the body with pressurized oxygen, hyperbaric oxygen therapy triggers a cellular signal that stimulates the permanent growth of brand-new capillary networks, restoring long-term blood flow to damaged tissues.

^ The bold portion above is a direct parallel to TB 500 (Frag 17-23)

• Here’s a screenshot of an old post I made about benefits of HBOT:

The below table explains how Fragment 17-23 signals cells to build blood vessels / improve blood flow and heal:

• Nerves / Nervous system dysregulation

• Brain inflammation

• GI inflammation

• Systemic, body-wide issues

Features of Thymosin Beta 4 Fragments

A pdf version is here:

Download

Our Dosing

Our dosing is low, there’s room to go up if you have a serious / acute injury or just want to experiment.

I purchased a 5mg vial and reconstituted it with 10mL of water (shown below)

• The first day I used 2 sprays, twice a day under the tongue, for my tennis elbow (healed by next day)

• Since then I use 1-2 sprays – in the morning with food

• My daughter uses 1 spray

We use it approx 3-4 days per week, but daily use or higher doses is fine

We notice a bit of additional energy right after using it (dose dependent)

We haven’t noticed any side effects other than, similar to HBOT, too much or too late in the day can cause an alert brain.

• If you want it twice as strong – you can purchase the 10mg vial

Purchasing TB 500 (Frag 17-23)

I bought ours from Limitless Life

They are a USA company and we’ve trusted their products for years.

If you buy TB 500 somewhere else, please verify it’s specific fragments and testing certificates.

Note: Like many peptide companies, Limitless recently began requiring signing into an ‘account’ to shop

Anyone can set up a free, basic account to sign in.

Don’t let the compliance certification phrase deter you, it’s there to prevent regulatory issues – Everyone is a “research professional”

•••

• Link to Fragment 17-23: https://limitlesslifenootropics.com/product/tb-500-17-23/

Selection FAQ: 5 or 10 mg is strength not size, Premium or to save money, Non-Lyo is ok too (difference here)

• Spray Kit: To use as a spray, you’ll need a sprayer & water (a syringe to transfer is included too): https://limitlesslifenootropics.com/product/sprayer/

• Coupon code: QUALITY takes 20% off everything — plus, there’s a markdown 20% sale now that stacks with the code for a double discount)

~ QUALITY Code never expires, sale ends 10/21/26

To buy Fragment 1-4

If you want to stack TB4 Frag 17-23 with 1-4, you can add it to the same sprayer or take separate

• TB4 Fragment 1-4, Vial for spray: https://limitlesslifenootropics.com/product/tb-500-1-4/

•••

• TB4 Fragment 1-4, Capsules:

We use powder under the tongue and use approx 1/4 capsule or less per day.

– Available from Limitless (here)

– Integrative Peptides (their capsules are acetylated and amidated which enhances availability (here)

Frag 17-23 is not available in capsules

•••

Note: For anyone with active cancer – Thymosin Beta 4 peptides especially (17-23) and also HBOT help with angiogenesis (blood vessel growth & support)

This healing benefit is excellent for most people – but strong blood vessels can also help cancer cells survive

If you avoid cell nourishing supplements or treatments like HBOT (as a way to starve off cancer cells) these would be ones to skip

Please know, this is does not mean any of these things cause cancer

While you’re Shopping — What else do we like from Limitless?

• You can find links to other Limitless Life products we use at this page

Notably, with links to our posts: Larazotide Acetate (Gut tight junctions), Oxytocin (social anxiety), Selank (anxiety), DSIP (sleep), 5Amino 1 MQ (fat cell metabolism), ARA 290 (nerve repair) but there are others and they’re listed at the link above.

• To learn more about any of the peptides we use, you can find articles, videos, search tool, AI tool, and more at our peptide resources page 🧬

Reconstitution (assembling the spray)

When your dry peptides arrive you’ll need to transfer them into the spray bottle

•••

Note: Yes, we know the sprayer is a nasal spray bottle ☺️

Our community (FB group with thousands of members) found unanimously that using short chain peptide spray used sublingually is equally effective to intranasal use

You can confidently use the sprayer any way you prefer!

•••

Reconstitution is not difficult and we have guide with a video here:

Also here’s a handwritten overview:

This is the same for any peptide in spray version

Thymosin Beta 4 wrap up

These fragments of TB4 are ones I wouldn’t want my medicine cabinet to be without!

Whether using them for acute healing needs or longer term for chronic concerns and inflammation, we’ve witnessed them to be incredibly effective.

I’d definitely want to give them a try before investing in HBOT if I were to do it over again and the peptides were available 😌

While I can’t give an age range for use, I can say that in our Recovering Kids Group, parents have reported MAPS doctors indicating both fragments for their young children.

If you decide to give Thymosin Beta 4 a try, I hope it can bring you / your family much healing too!

Throwback from our HBOT era 🥹

For all of our other peptide content (blogs, videos and more) you can see:

Thank you for stopping buy!

🤍

Nothing at this site is medical advice. Always consult a trusted medical advisor (which could be yourself) and research before starting any supplements.

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