Reconstituting Peptides

To receive what you need, you must order products as the spray version.
If you use links from this page – they take you to the right products.

If you have a spray you need to reconstitute, don’t worry – You’ve got this!

Parts you need:

These are included with Spray Version orders

  • Peptide in a vial
  • Deionized water (10mL vial) OR saline (two 5mL ampules)
  • Syringe (If you need extra, we use these)
  • Nasal Spray bottle (which we also use sublingually)

Note: If you’re not using your peptide as a spray or drops, please see this page.

Storage before reconstituting:

You will find varied advice, but keeping them at room temp for up to 2 years (lyophilized) a bit less if non-lyophilized – has worked well for us and many others. Freezing is sometimes recommended but not required.

For Lyophilized products

• Watch the video

• Read written instructions at the bottom of the page, if needed.

• Always refrigerate reconstituted sprays (they are only peptides and water – no preservatives)

• Lyophilized is also called “premium” on the Limitless Site

Non-lyophilized products

From Limitless: These are now also in a small vial and you will follow the above. If you have older products (with no separate small vial) see below for different instructions.

• Refrigerate after mixing.

Reminder – these products work equally well sublingually, see poll at bottom of page.

Video:

Step by step live demo for limitless spray reconstitution

You can skip past the intro & sublingual use demo to the reconstitution (01:30 min mark)

Tip!

If you inject air of an equal amount as you wish to extract into the vacuum sealed bottles it will make extraction much easier.

This is shown in the above video.

How to order any dry peptide vial and DIY it into a nasal spray

Note: These vials are not powders that can be used like a powder supplement, they are highly concentrated and need to be reconstituted in liquid.

• On the Limitless website you can purchase products that are not designated as spray, sold as a vial only.

• You can make them into a spray by simply ordering the sprayer kit separately.

This is explained also on our buying page: https://diaryofrecovery.com/buy

Here is what you receive when you order the “sprayer”

Limitless Instructions

Non-Lyophilized Versions

Please see the note (above, blue section on left) that some bottles appear empty.

– In the past, this version was already in the spray bottle and you will not have a separate small vial.

– Now, they are sent in a separate vial so there is no difference but your vial may look empty except for fine dust.

When you have no separate small vial, simply pour the deionized water into the spray bottle.

Biolab Instructions

Below are their written instructions, but you will follow the same video above.

Only difference – Instead of a 10mL glass vial of deionized water, you will receive two 5 mL saline plastic ampules.

They are interchangeable.

This very short video is only to show the product differences:

Differences for Biolab reconstitution

You still need to watch the full reconstitution video above 😌

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FAQ

I left my reconstituted spray out of the refrigerator one night overnight, is it ok?

For that short of a time it’s more about the bacteria than the peptide integrity — There are no preservatives.

If you’re using it as a sublingual spray that doesn’t matter as much.

As a nasal spray, it could matter more.

Also, if it’s consistently unrefrigerated the peptides will degrade more quickly.

All that said, you’re probably ok for one night.

Do I need to refrigerate or freeze the vial for storage before mixing?

No you don’t – room temp is how Limitless suggested to store, until recently they changed it to say freezing may be slightly better for long term (beyond 6 months+)

Refrigerators are humid so they are not recommended until after reconstitution.

Never freeze a reconstituted spray.

Where can I get a syringe for mixing?

If you didn’t receive one with your order, or need a replacement, you can order from Amazon here: https://a.co/d/03jH27aY

Reminders about ordering:

If you follow links from our buy page you will always have the correct products: https://diaryofrecovery.com/buy

These links take you to the “spray” versions. The image you see will look like a sprayer.

If you order peptides as vials, you will not receive what you need to reconstitute the product.

• Also note: at limitless – You never need to add the “people also buy” items, such as an empty vial. Please ignore it when those pop up.