Shelf life of peptides

After mixing we have had nasal sprays still potent after a year (Selank, oxytocin, DSIP)

Capsules 2-3 years minimum

From the Infographic:

Use of Peptides for Healing – a Common Sequence (must tailor to symptoms, needs)

Step 1 – NAD+ and/or Plavelle (System wide Support)

Start with a cellular energy foundation. NAD+ supports mitochondrial function, improves cellular repair, calms mast cells & assists detox pathways. Starting here prepares cells to handle antimicrobial and tissue-repair work.

1 spray sublingual, sometimes less. diaryofrecovery.com/mito

Plavelle can also be a gentle first step, a body wide bioregulator for healing, the immune system and gentle antimicrobial work. diaryofrecovery.com/plavelle

1/4 cap 2-3x per day for 1 month, less second month, taper and maintenance dose as needed

Step 2 – Selank (Anxiety & Nervous System Regulation)

Use Selank early to calm anxiety and calm the nervous system. This helps tolerate subsequent antimicrobial actions and reduces stress-related inflammation.

Sublingual use works well. Selank can reduce anxiety, and blunt exaggerated inflammatory responses during any future microbial die-off.

1 spray 1x daily, can go up to 3x per day diaryofrecovery.com/selank

Step 3 — Larazotide (Close Tight Junctions)

Close and support gut and brain tight junctions during exposures to food or other irritants / toxins including die off from KPV. Helps contain die off products in the gut and reduce systemic leakage that can worsen symptoms.

Use Larazotide to reduce translocation and neuroinflammation risk. Works only while taking it so combining with KPV is ideal. diaryofrecovery.com/larazotide-acetate-info

1/6 – 1/4 cap just before meals or with KPV dosing

Step 4 – KPV (Antimicrobial, Antifungal, Anti-inflammatory)

Ideal to Introduce KPV after cellular, nervous system, and barrier supports are in place.

KPV offers antimicrobial and mast cell-calming benefits and excellent inflammation reduction.

Dosing: Start very low 1/20th cap and dose frequently (up to every 3 hours)

Expect possible die-off after several days. Keep NAD+ Selank and Larazotide to reduce symptom severity. diaryofrecovery.com/kpv

Step 5 – TB4-Frag (Tissue Repair & Healing)

Once microbes are more controlled and barriers supported, add TB4-Frag to accelerate tissue healing and support remodeling. TB4-Frag can complement antimicrobial steps by further promoting repair.

diaryofrecovery.com/tb4info

Use TB4-Frag for healing from all types of tissue damage, including from injury or toxin exposure.

1/4 cap 2-3x per day as needed

Step 6 – Single-Organ Bioregulators (Targeted Support)

Begin Anytime – 26+ effective Organ / Gland specific bioregulators to amplify and focus healing. For Full List, See: diaryofrecovery.com/tinypeps

Examples: CNS / Brain bioregulators – cognitive support and neurorepair • Liver & kidney bioregulators – detox and metabolic recovery • Gastric Mucin – Acid reflux & Intestinal repair • Adrenal & HPA axis – stress resilience and hormone regulation • Pineal gland bioregulators – circadian support and anti-aging. • Prioritize organs needing targeted rejuvenation.

Except for time of day specific hormone needs, typically 1/4+ capsule 2-3x per day for first month, less second month, maintenance dose as needed.

Step 7 – DSIP for Sleep (Add Anytime)

DSIP can be added at any stage to support deeper more restorative sleep, critical for immune function and tissue repair. diaryofrecovery.com/dsip

Improved sleep helps recovery from die-off and enhances the effects of other peptides.

Introduce DSIP when sleep is disrupted or during phases of intense healing. 1-2 hours before bed. Experiment between 1/2 to 3 sprays, dosing is very person dependent.

Clarify policy: We support nasal sprays for Selank and DSIP, and we also note sublingual as a workable option.
Give the correct basics
• Selank: about 1 spray as needed for calm, up to a few times per day.
• DSIP: about 1 to 2 sprays in the evening, timing by feel, often 1 hour before bed.
All dosing is approximate and adjustable.

———

Why do people pulse dose? With the peptides that we use it’s not because they stop working

It’s typically one of two reasons:

They just aren’t needed continuously (repair has happened and / or genes have shifted and results can stick for a while)
- In this case, the breaks aren’t “required” and there wouldn’t be harm from continual use. But to save cost and effort it’s best to see how long you can go and remain symptom-free.
They work too well and taking them without breaks is too much. (Brain Bioregulators can be too Stimulating or DSIP can cause daytime tiredness, KPV killing too much yeast or bacteria)

calm-at-a-glance summary of Eckhart Tolle’s core teachings you can use as a nervous system reset:

Presence: Come back to this moment. Feel your breath, hands, feet, sounds around you. The body registers “safe” when attention is here, not in stories about past or future.
The pain-body: Emotional pain can accumulate and flare. Notice it as sensation and energy in the body instead of feeding it with thoughts. Name it gently, breathe, let it pass through.
Watch the thinker: You are the awareness behind thoughts, not the thoughts themselves. When you observe a thought, it loses some charge and the body settles.
Acceptance: Let this moment be as it is, then act from clarity. Inner acceptance lowers resistance, which lowers sympathetic drive.
Conscious breath: One slow inhale, longer exhale. Feel one full breath from start to finish. Repeat a few times to anchor presence.
Space between thoughts: Even a second of quiet counts. Rest there. That gap is the nervous system’s sweet spot.
Surrender vs. passivity: Surrender is saying yes to “what is,” then choosing your next step. It’s active, not giving up.
Inner body awareness: Place attention inside the body (hands, chest, belly). Subtle aliveness is a direct path out of mental noise.
Stillness in daily life: Use micro-pauses at doors, before emails, while washing dishes. Tiny moments of presence add up.

Quick practice you can do anywhere:

Feel your feet on the ground.
Soften your jaw and shoulders.
One conscious breath with a longer exhale.
Notice one sound, one sight, one sensation.
Say silently: “Only this moment.” Stay for two more breaths.

With my coaching clients, I almost always have them start with NAD+ 1st

just because it’s listed last on some of the webpages. It doesn’t mean that it is less important. I think that they can help calm down the body and get them ready for peptides.

Q. Why do some people prone to acid reflux get reflux from taking KPV under the tongue (or spray) but not when they take it in another capsule – same size dose ?

A: our theory – KPV isn’t irritating in the way an unpleasant chemicalical or abrasive powder would be.
Instead, because KPV is such a powerful, immune and inflammatory signal peptide, when it hits the delicate mucosal lining under the tongue and upper throat, it might be activating or calming certain cells in a way that temporarily affects the local nervous system or digestive reflexes.

🍃Clean floors are often best for allergy-heavy homes.

People with acid reflux often have a hypersensitive esophagus or a slightly dysfunctional lower esophagal sphincter. The moment KPV contacts the nucosa, it could be triggering a reflex, kind of like a signaling flare that leads to immediate sensations of reflux, or a change in the way the acid moves upward, even if the gut itself isn’t irritated yet.
So it probably isn’t classic die off happening in real time, and it isn’t direct irritation that damages the tissue. Rather, it’s KPV activating local receptors that influence the reflux response reflex pathway. You might call it a neuroimmune reflex effect tied to a more sensitive individual’s esophageal lining.
Capsuling KPV buffers this reaction because the peptide doesn’t hit those nerves and nucosa first, it passes into the stomach, where it can do its healing and antimicrobial work without triggeringgering that immediate protective reflex. This also fits with the idea that KPV’s benefits come mostly after gene expression and tissue healing take place over days to weeks, rather than immediate chemical irritation or toxicity. It’s a nuanced difference, but an important one that honors both what you’re seeing and what science tells us about how this peptide works.
I wish I had a perfectly clean cut, simple explanation for the exact cellular mechanism. Honestly, I don’t. But based on our combined understanding that neuroimmune local signaling is the most plausible piece to the puzzle right now, it respects the fact that it’s not the powder itself or the peptide burning tissue, but rather a highly sensitive reflex being triggered in some folks under specific conditions.

Q: with low serotonin that (oral) collagenfragments can create more imbalance & depression. Do you know if this applies to the Collacell peptide as well?

the bioregulator, Collacell, is nothing like taking oral common collagen “peptides” – i had bad results from taking collegen peptides MANY years ago (mood issues)

I love Collacell! All it does is help your body improve skin elasticity and joints, no possibility of an imbalance

——
Previous message:
🔮Selank Déjà vu – it’s NOT sold out yet —

You can still order the vial with the sprayer kit and have the exact same product sent to you as if you ordered it as a spray. 👏🏻

Here’s how… go to https://diaryofrecovery.com/buy (Watch the video if you want even more of a walkthrough of how this works) 🙂

••••••••Also: yes, they are all ok to buy: lyophilized, non-lyophilized, 99+% 99% 98.5%

We have used them all. They are fine 🥰 if you want to save money, order the lowest price one, they all work ❤️

sublingual use: spray under the tongue or dilute in VERY small amount of water – see below.

Sublingual Sprays – Not just for kids!

My daughter and I have been experimenting with sublingual use for Selank & DSIP Sprays (Same nasal spray, used under the tongue, sprayed or as drops)

OBSERVATIONS:

It works amazingly well! I would say equal to nasal dosing but because of variables of life, I don’t want to make that statement unequivocally 🙂

I’m so surprised that the standard mantra (outside of this group) is that they don’t work. They clearly work!

Some people here say it works even better than intranasally
Next question — Is it more convenient to use the spray under the tongue or to transfer the peptide to an eye dropper bottle, and any issues with that?

——-Here’s what we do: ——-

For full sprays (Selank) just spray under the tongue, being careful not to tip the bottle too far upside down

For half sprays (DSIP – all I need now is 1/2 spray… note: the limitless DSIP is much stronger than the Biolab version):

I initially sprayed it into a cup and diluted with a very small amount of water (~20 drops) then took half each person.
Now that I know sublingual is our preferred method, I’m trying drops.

💧It appears that 2.5 drops = 1 spray (per much research and observing)

🙋🏻‍♀️ Q: What method do you use and any observations or tips to add?

I doubt I’ll go back to using any peptides as nasal spray – there seems to be no need 🤷🏻‍♀️

And I’m feeling sorry to everyone that I didn’t figure this out earlier! 😵‍💫

🫁🫀 thought it might help if I wrote a quick list of some benefits we see from each of the Vita Peptides 🧬

Also they’re all now in the smaller size too! (Including Adrenact) ••• see 1st comment

Here goes 🫶

Adrenals – excellent during illness, stress, for emotional regulation, or in menopause (adrenals take over hormone production as ovaries step back) ✋🏼🫠😬
Bile – if you have watery stools or urgency, consider bile acid malabsorption as a cause & if so, this can help 🙏🏼 (gall bladder supportive)
Bone Marrow – helps me with red blood cell production / anemia (with b12) 🩸
Cartilage – great for my aging joints 🦴
Collagen – skin elasticity & joints noticeable changes – a favorite bioregulator! 🌸 (note, Collacell is not like taking ‘collagen peptides’ – its a bioregulator, helps your body produce more of its own)
Gastric Mucin – cured daughters 3 year battle w/ acid reflux 🏅 (heals & prevents ulcers too)
Heart – my heart feels stronger, I can exercise longer 🫀🛼
Hypothalamus – hormone regulation & stress 🍃
Kidney – helps with detox! (prevents stones and uric acid buildup when I eat animal protein) 🥩🍗
Lungs – My lifetime of allergic asthma is gone! (Also microdosing KPV) I absolutely owe my quality of life to these two things! 🫁🏆
Ovaries – excellent for peri & menopause! Noticeable changes. (See over 40 post) 💕
Pituitary – stress buster! I love it & supporting HPA axis reduced my seasonal alleriges even w/o KPV (hypothalumus – pituitary – adrenal) 🧘🏼‍♀️
Prostate – reduced nighttime waking for men 🚰
Stomach – helps with digestion 🍔 (and ulcer healing / prevention)
Testes – muscle mass boost for men 💪🏼
Thymus – this or Thymogen for acute illness – Thymogen is better 😉
Thyroid – ThyroPep is better 😉

❤️❤️

Social Butterfly?! Oxytocin

Yesterday we had a social outing with people who in the past have typically caused my daughters anxiety to spike to the point to where she couldn’t enjoy herself.

Recently she’s been helped by 1 spray of Selank and 1 spray of Oxytoxin.

As an experiment, I decided to try 2 sprays of oxytocin (plus 1 of Selank) to see if there was a difference. (All sublingual)

And WOW there was! She was completely calm for 2 1/2 hours, not asking to go home (where usually she would be) and notably more comfortable, interacting with others to the extent everyone noticed 💞

I used Biolab brand 10 mg oxytocin (which we’ve had in a refrigerator for over a year because we don’t use it that much – so that shows you its shelf life)

To compare it to Limitless, normally Limitless products work better, but theirs is only 5 mg

If you want to try to experiment with more, I would probably start with:

3 sublingual sprays of Limitless Oxytoxin

To test the theory that it’s best to use oxytocin only intermittently, I gave it to her again today

She is very calm, but not as socially outgoing as yesterday – of course there are other variables involved – but the studies I read do suggest that oxytocin works best when used intermittently.

To me that would mean at most three days a week, but I have not seen any actual guidelines for frequency— It would just require experimenting.

If you want to read a little more about oxytocin, I will put the link in the comments

Also where to get it from either company, but I don’t think biolab is shipping to the United States currently, I could be wrong.

Someone can let me know!

Hoping this can help others find their wings too! 🦋

oxytocin

Shelf life info (spray) lasts a long time!

At 2°C- 8°C (refrigerated) shelf life of 3 years.
Below 21°C / 70°F shelf life of 2 years.
At 25°C / 77°F the shelf life is reduced to 1 year.
At 30°C / 86°F the shelf life is reduced to 6 months.
At 40°C / 104°F transport – maximum 1 week.

Leucovorin for Autism: Real‑World Wins, When It Backfires, and How to Balance It Safely

Leucovorin (folinic acid) in plain language

Leucovorin is folinic acid, an active folate the body can use right away. It’s popular among autism parents because it supports methylation—a key biochemical process that helps repair DNA, regulate brain function, and control detox pathways.
Many kids on the spectrum struggle in these pathways. Folinic acid can feel like a smart shortcut to boost neurological function and sometimes improve speech, behavior, or energy. That’s why it’s become a go‑to.

Why it helps some kids (and stirs others)

Leucovorin isn’t a magic bullet. Some children show striking gains: clearer speech, calmer behavior, steadier energy. Others hit the “flip side”: agitation, anxiety, worse sleep, or amped‑up behaviors.
A big reason for that flip: genetics that change how methylation and neurotransmitters are handled. Variants in COMT or MTHFR can make a child methylation‑sensitive. Kids with “slow COMT” don’t clear dopamine quickly; too much methyl support can overstimulate them, showing up as irritability, racing thoughts, or insomnia.
These reactions sometimes get labeled “detox” and brushed aside. In reality, many kids are methylation‑sensitive, and common folinic dosing can push them past their safe range if other supports aren’t balanced.

Why balancing nutrients matters

Without the right B‑vitamin forms alongside folinic acid—especially the right B12 form for that child, plus B6 (P5P) and natural antioxidants—the methylation cycle can get thrown off. That can drive more oxidative stress and nervous system imbalance instead of the calming and clarity parents are hoping for.

Testing that helps tailor the plan

If you’re diving into Leucovorin, pausing to check genetics is smart. Two common contributors in autism: COMT (especially Val158Met) and MTHFR variants. These can be assessed through consumer services like 23andMe or via clinical genetic testing panels ordered through functional‑style clinics or labs. Knowing your child’s COMT/MTHFR picture can guide whether to use methyl B12 (methylcobalamin) or gentler forms like hydroxocobalamin or adenosylcobalamin.

Gentler B12 strategies when Leucovorin causes side effects

Start low, go slow; avoid rushing methyl donors in sensitive kids.
Hydroxocobalamin can support detox and nervous system health with less risk of overstimulation than methylcobalamin.
Adenosylcobalamin supports mitochondrial energy, often helpful where fatigue or specific gene issues are in play.
Keep B6 in the active P5P form to help enzymes work without pushing the system.

Peptides and supports that complement or replace Leucovorin

CogniPep: supports brain and vascular health, improves cognition and calms neuroinflammation without the jittery feel some vitamins can cause. Parents often see better language, focus, and motivation.
KPV: calms immune activation and gut inflammation; in many families, gut and immune calm correlates with fewer behavioral flares.
NAD+: steadies mitochondria and cellular energy, which can smooth crashes and lower neuro‑immune irritability.
Used together, these can target the same big systems Leucovorin aims to help—brain, immune, gut, energy—in a more contained, measured way.

When to pause Leucovorin

Red flags of overstimulation: anxiety spikes, worse sleep, more intense or rapid‑cycling behaviors.
Practical pivot: pause Leucovorin, add balancing supports like hydroxocobalamin and the peptides above, then reassess. Many kids “reset” with this approach.

What I want parents to know

Leucovorin isn’t “good” or “bad.” It’s a tool. It can help, but it needs respect for individual genetics and sensitivity.
Think of it as one option inside a broader toolkit. Balance methyl donors with supportive B‑vitamins and peptides. Never push dose without watching closely.

Alternatives when Leucovorin isn’t a fit

Hydroxocobalamin or adenosylcobalamin for B12 support.
Active B6 (P5P) as an enzyme helper without overdriving methylation.
CogniPep and KPV to cover brain, immune, and gut stability.
NAD+ for gentle, steady energy so lows don’t trigger flares.

Testing sources beyond 23andMe

In addition to 23andMe, parents can use clinical genetic testing panels offered through many integrative or functional health clinics and standard clinical laboratories. These panels commonly report COMT and MTHFR variants and can be ordered and interpreted within a clinical setting.

Quick‑start, watch‑and‑adjust checklist

If trying Leucovorin: start low; pair with the right B12 form for your child; include B6 (P5P) and gentle antioxidants; monitor for stimulation.
If stimulation appears: pause Leucovorin; shift to hydroxocobalamin, consider adenosylcobalamin; layer in CogniPep, KPV, and NAD+ to stabilize brain, gut, immune, and energy.
Reintroduce only if and when your child feels steadier, and still go slowly.

This is exactly how I walk families through it: keep the wins, remove the friction, and let your child’s nervous system be your guide.

Member Question: Is there any way to have rugs safely around our sensitive kids?

Answer: Rugs and Allergies: What Helps, What Hurts, and When to Skip Them

The Core Problems

Fibers: most react to wool and jute; many natural fibers still carry lanolin, plant dust, or sizing agents that can irritate.

Chemicals: synthetics like nylon, olefin, and acrylic often come with VOCs, stain guards, and synthetic fragrance from manufacturing or storage.

Dust mites: rugs trap skin flakes and humidity, which feed mites. Their feces are the main allergen and settle deep in pile; daily vacuuming is usually required and doesn’t capture it all.

Ongoing load: even “natural” rugs can shed fibers and dust. If you’re already reactive, that background load can tip you over.

Why dust mites are hard to beat in rugs

They thrive in fabric + humidity. Area rugs act like mini-carpets, storing dust daily.

Standard vacuums miss a lot. Even with HEPA, you often remove surface dust but leave embedded allergen.

Washing is limited.

Most rugs can’t be hot-washed or fully dried often enough to control mites.

If you must have a rug, make it as tolerable as possible

Choose materials: tightly woven organic cotton or flat-weave cotton/linen with no wool, no jute, no sisal, no blends. Avoid acrylic and “soft touch” synthetics that off-gas.

Construction: flat-weave or low-pile only. No shag, no high pile, no thick pads. The less loft, the less mite habitat.

Backing and pad: pick natural rubber (but these have odor issues of their own) or untreated felt pads that are low-VOC. Skip foam pads and anything “odor resistant” or “stain-proof.”

Sizing: go smaller so you can launder or take it outside easily. Runners or small mats beat giant area rugs.

Pre-airing: unwrap outdoors, air in sun and breeze for several days before bringing inside. Sun helps off-gas and reduces some surface microbes.

Cleaning:

Vacuum with a sealed-HEPA machine slowly, ideally 2–3 passes, at least twice weekly but daily if tolerated.

HEPA machines still spill dust mite allergens back into air and stir them up in the room.

Take rugs outside monthly to beat out dust; sun both sides.

If the rug is truly washable, hot-wash and fully sun-dry. If not washable, that’s a signal it may not be suitable for a highly allergic home.

Humidity control: keep indoor RH around 40–45 percent to make life harder for dust mites.

Placement: keep rugs out of bedrooms and near seating where you spend long hours. If you want one, try an entry mat you can wash often.

Clear no-go’s for sensitivities:

Wool in any form, including “washable wool.”

Jute, sisal, seagrass, coir.

Acrylic or “poly blend” with stain-guard or antimicrobial coatings.

High-pile, shag, or tufted with latex glues that off-gas.

When “no rug” is the healthiest answer:

If reacting to both natural and synthetic fibers, or can’t maintain twice-weekly HEPA cleaning and monthly sunning, hard floors with washable cotton runners or no rugs at all are the calmest choice.

A workable middle path, if you want to test:

Start with one small, flat-weave organic cotton runner in a low-traffic area.

Air it outside for several days first.

Use it for a week, track symptoms, then decide to keep or remove.

If it’s fine, consider a second small piece. If not, return to no-rug.

If a rug is important for comfort or acoustics, flat-weave cotton, very small, fully washable, and aggressively maintained is the only setup consistently tolerated.

#rugs #allegens

At-Home Zonulin Stool Test (No Doctor Needed)

You can totally order these direct-to-consumer kits online—they’re easy, private, and mail-in. Here’s how to get started and what to expect:

1. Search and Order: Google “zonulin stool test kit” or “leaky gut zonulin home test” on sites like Amazon, or go straight to lab sites (e.g., Verisana, Doctor’s Data via TrueHealthLabs, GetTested, or IFDW). Prices are usually $150–$300, including shipping.

2. Popular Kits to Try (all mail-in, no doc required):

• Verisana Zonulin Stool Test (on Amazon): Collect a small stool sample at home, pop it in the prepaid envelope, mail to their CLIA-certified lab. Results emailed in 2–3 weeks as a detailed PDF report.

• Doctor’s Data Zonulin Stool Test (via TrueHealthLabs.com): Similar process—kit ships to you, sample collection takes minutes, mail back. Results in about 2 weeks via secure email; 96–98% accurate via ELISA method.

• GetTested Leaky Gut Test: Measures zonulin specifically; kit includes everything for easy stool sampling. Mail in prepaid envelope—results in 10–14 days by email.