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Link to video (the organization that recorded it requires a free registration):
Summary of the Video & Slides
Overview and Goals:
• Dr. Holtorf aims to help attendees understand oral peptide bioregulators, focusing on their benefits and limitations for longevity and aesthetics, emphasizing practical study results over theoretical details.
• Key bioregulators discussed: thymus, pineal, thyroid, brain, skin, muscle, pancreas, prostate, testes, adrenal, liver, and vascular.
• Peptide bioregulators are short chains (2-4 amino acids, ~500 daltons), purified for high potency (5x more active than standard 10,000-dalton extracts), and tissue-specific, targeting organs like brain, heart, or skin.
• Unlike peptides (many only act on cell surface receptors) or hormones, bioregulators enter cell nuclei, binding to DNA (histones, activators, repressors) to upregulate healthy genes and downregulate disease/aging genes.
Immune System and Aging:
• The immune system is central to longevity, healthspan, and reducing chronic illness, with two components: TH1 (intracellular defense, e.g., cancer, infections, autophagy) and TH2 (extracellular defense, inflammation).
• Thymus involution (starts ~age 15, significant by 30, low by 40-45) shifts the immune system from TH1 to TH2, causing “inflamm-aging,” mitochondrial dysfunction, and increased disease risk (e.g., cancer, chronic illnesses).
• Per the CDC, 80% of aged individuals have at least one chronic illness due to thymic involution, costing lives and resources.
• Stress, toxins, pesticides, chronic infections, and vaccines exacerbate inflammation, mitochondrial dysfunction, and “turbo cancers.”
• Thymic peptides (e.g., Thymogen Alpha-1, KPV) restore immune balance, enhance natural killer cell function, and reduce biologic age. Oral thymic peptides are ~100x more potent than injectable thymulin.
• Benefits include improved outcomes in chronic fatigue syndrome, Lyme disease, fibromyalgia, and multi-system illnesses by modulating the immune system, reducing the need to treat individual symptoms.
• Studies like the HIT trial aim to rejuvenate the thymus, but thymic peptides are cost-effective (“pennies a day”), safe, and effective.
• Labs (e.g., natural killer cell function, TGF-beta, C4A, CD4/CD8 ratio) can assess immune function and biologic age.
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More on Thymus:
• Thymus involution and TH1-to-TH2 shift (with T-regs and TH17 involvement) trigger a “cell danger response,” where mitochondria produce reactive oxygen species (ROS) instead of energy, increasing inflammation (SASP).
• This causes pineal, hypothalamic, and pituitary dysfunction, leading to central hypothyroidism or low testosterone (missed by standard labs due to normal TSH or low-normal ranges, e.g., testosterone at 200-300 vs. optimal 500-900).
• Solutions include bioregulators, peptides, medications, or senolytics to clear senescent cells, reducing inflammation and multi-system illness.
Historical Context of Bioregulators:
• Developed during the Soviet Union to address rapid aging in cosmonauts, jet pilots, and submariners, under Colonel Khavinson, who collaborated with Pavlov (known for conditioning but also peptide research).
• Thymic and pineal peptide extracts from young calves reversed rapid aging, initially kept secret for military and Olympic use.
• Post-Soviet Union collapse, Russian studies were published but initially overlooked due to language barriers; later gained attention in the U.S.
• Thousands of studies support bioregulators’ efficacy in extending lifespan and treating chronic illnesses.
Thymus Bioregulators:
• Reverse thymus involution, restore a youthful immune phenotype, and reduce biologic age.
• Improve chronic illnesses (e.g., Lyme, chronic fatigue, fibromyalgia) by enhancing TH1 function (natural killer cells, autophagy).
Effectiveness / Safety:
• KPV: antibacterial and anti-inflammatory while modulating immunity, unlike antibiotics & cortisone, which suppress it.
• Bioregulator are Extremely safe, with no toxic dose found at 1,000-10,000x normal doses.
Pineal Bioregulators:
• Increase telomere length, surpassing the Hayflick limit, and reduce cancer risk (e.g., 73% reduction in breast cancer in mice with epitalon, 5 days/week from month 2 until death).
• Improve melatonin levels (outperforming IV vitamins), reproductive health (8% irregular menstrual cycles vs. 52% in controls), and lifespan (up to 90% increase in animal studies).
• A 12-year study (15-year follow-up) with epitalon (6 doses) and thymic peptides in cardiovascular disease patients showed reduced mortality, cancer rates, colds/flu, and improved cardiovascular function, with greater effects when combined.
Thyroid Bioregulators:
• Normalize thyroid function: raise low levels, lower high levels, and reduce thyroid nodules.
• Thyroid axis bioregulators treat autoimmune thyroiditis (Hashimoto’s), significantly lowering anti-thyroglobulin and anti-TPO antibodies (unlike standard thyroid bioregulators).
• A study with 25 hypothyroidism patients (with cardiovascular disease and thyroid atrophy) showed 78% clinical improvement with thyroid bioregulators vs. T4 alone after 3 months, with benefits in fatigue, endurance, headaches, dizziness, chest pain, and angina after 20 days.
• Improve neurologic function, cardiovascular health (via vessel dilation), cholesterol, insulin resistance, metabolic function, hair, skin, endurance, and healthspan.
• A study showed thyroid axis bioregulators boosted thyroid levels post-pituitary removal.
Skin Bioregulators:
• Increase collagen production (up to 167%), sirtuins (SIRT1, SIRT6; 35-75% increase), and skin health markers (KI67, CD98; up to 650% increase).
• Reduce inflammatory markers (IL-1, NF-kB, TGF-beta) and senescent markers (caspase-3, MMP9; up to 73% reduction).
• 500 mg daily reduced wrinkles, increased elasticity, hydration, and skin whitening (via tyrosinase suppression and reduced melanin transport) over 12 weeks, with significant results by 4 weeks.
• Decreased menopausal elasticity & hydration symptoms and improved skin brightness (3-fold improvement by 12 weeks).
Muscle Bioregulators:
• Enhance physical performance after 2 months, with significant increases in:
• Grip strength (left and right hands), a key aging biomarker.
• Long jump performance (lower body strength).
• Step test performance (cardiovascular and muscular endurance).
• Flexibility (statistically significant).
• Reduce fatigue and speed muscle recovery, beneficial for athletes or active individuals.
• Tissue-specific, acting directly on muscle tissue.
Pancreas Bioregulators:
• Improve insulin resistance in type 2 diabetes (918 patients, Moscow study).
• Reduced fasting blood sugar and medication dependency compared to standard treatment, with greater benefits when combined with pinealon (subcortical brain bioregulator).
• Prostate and Testes Bioregulators:
• Prostate bioregulator improves benign prostatic hyperplasia (BPH).
• Combined prostate, testes, adrenal, and liver bioregulators synergistically boost testosterone and libido (less effective if any are omitted).
Ovaries and Women’s Health:
• Ovary Bioregulators in 135 menopausal rats restored menstruation in 85% and live births in 25%.
• In women, increased anti-Müllerian hormone (AMH; 68%), ovarian reserve (98% increased antral follicles), and pregnancy rates (71% after 4-8 months, 84% overall in women unable to conceive, even with IVF).
• Reduced menopausal symptoms and improved FSH/estrogen balance (estrogen from 49 to 243).
Vascular and Other Bioregulators:
• Vascular bioregulators enhance other bioregulators’ efficacy, reduce endothelial dysfunction, and lower SASP.
• A study with 416 cerebral atherosclerosis patients using vascular, pineal (subcortical brain), liver, and pineal bioregulators (4 months) reduced cholesterol, triglycerides, and improved quality of life.
• Brain bioregulators improved neurodegenerative symptoms (e.g., Parkinson’s, Alzheimer’s, post-stroke) in 65% of patients vs. 27% with standard treatment, reducing headaches, sleep issues, emotional instability, memory issues, and fatigue.
• brain and vascular/cardiac/pineal bioregulators lower inflammation from dysfunctional mitochondria.
Clinical Studies on Biologic Age:
• A Russian study (100+ participants) using 5-7 rotating bioregulators (21 types, every tissue) reduced biologic age by 22 years (telomere length) over 3 years and 14 years (methylation age) over 5 years, lowering mortality risk by 50%.
• Being 4 years over biologic age increases mortality by 50%; 7 years younger reduces it by 50%.
• Dr. Holtorf’s program guarantees a 5-year biologic age reduction, measurable via methylation, telomeres, or biologic markers.
Safety and Practical Use:
• Oral bioregulators are extremely safe, with no toxic dose found at 1,000-10,000x normal doses, unlike some injectable peptides.
• Typical doses (e.g., 500 mg daily for skin bioregulators) are bioavailable and effective for anti-aging and aesthetics.
• A survey showed some practitioners already use peptides, with growing interest in bioregulators.
Personal Anecdote:
• Dr. Holtorf credits bioregulators with reversing their heart failure, fatigue, and fibrosis (caused by high TGF-beta) during medical school, after standard treatments failed.
• After taking peptides in Belgium, Dr. Holtorf could walk upstairs within 3 days, with heart function normalizing, transforming their practice for treating complex patients

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